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Multiple elements and auto-repression regulate Rox1, a repressor of hypoxic genes in Saccharomyces cerevisiae
J Deckert1, R Perini, B Balasubramanian
1Department of Biological Sciences, State University of New York at Albany 12222, USA.
Genetics
|March 1, 1995
Summary
The ROX1 gene product, Rox1, represses its own expression in yeast, a process involving its binding to its upstream region. Additional repressors and the Hap1 protein also regulate ROX1 expression under varying conditions.
Area of Science:
- Molecular Biology
- Yeast Genetics
- Gene Regulation
Background:
- The ROX1 gene encodes a repressor protein crucial for regulating hypoxic genes in yeast.
- Understanding the autoregulation of ROX1 is key to deciphering yeast's response to oxygen levels.
Purpose of the Study:
- To investigate the autoregulatory mechanism of the ROX1 gene.
- To identify factors involved in the repression of ROX1 expression.
Main Methods:
- RNA blot analysis
- ROX1/lacZ fusion constructs
- Gel-retardation assays
- Gene overexpression studies
Main Results:
- Rox1 was found to repress its own gene expression by binding to its upstream region.
- Overexpression of Rox1 inhibited yeast cell growth.
- Hap1 plays a role in both heme-induction and potential heme-absence repression of ROX1.
- A second repressor, dependent on Tup1/Ssn6, also controls anaerobic ROX1 expression.
Conclusions:
- ROX1 exhibits negative autoregulation, a novel finding for this repressor.
- Multiple regulatory factors, including Hap1 and the Tup1/Ssn6 complex, contribute to the complex regulation of ROX1.
- These findings provide insights into yeast's adaptation to hypoxic environments.