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Functional analysis of complement receptor 1 using a new monoclonal antibody, KuN241
J M Mathew1, B Naziruddin, B Duffy
1Department of Surgery, Washington University School of Medicine, St. Louis, Missouri 63110.
Hybridoma
|February 1, 1995
Summary
A novel monoclonal antibody, KuN241, targets complement receptor 1 (CR1) on immune cells. KuN241 binding to CR1 and Fc gamma RII triggers a unique transmembrane signaling pathway.
Area of Science:
- Immunology
- Cell Biology
Background:
- Monoclonal antibodies (MAbs) are crucial tools in immunology.
- Complement receptor 1 (CR1) plays a role in immune cell function.
- Transmembrane signaling pathways are critical for cellular communication.
Purpose of the Study:
- To characterize the antigen recognized by the MAb KuN241.
- To investigate the signaling mechanism induced by MAb KuN241 binding.
Main Methods:
- Immunoprecipitation and cell surface expression analysis.
- Functional assays measuring intracellular calcium ([Ca2+]i) levels.
- In vivo and in vitro cell activation studies.
Main Results:
- KuN241 recognizes complement receptor 1 (CR1) on monocytes, PMNs, B cells, and T cells.
- CR1 expression is downregulated on B cells after PWM activation and on PMNs/monocytes after PMA treatment.
- KuN241 binding induces a transient increase in intracellular calcium ([Ca2+]i) in PMNs and monocytes, mediated by dual binding to CR1 and Fc gamma RII.
Conclusions:
- KuN241 is a specific MAb for CR1.
- A novel transmembrane signaling mechanism involves dual binding of KuN241 to CR1 and Fc gamma RII.
- This signaling pathway offers new insights into immune cell activation.
