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Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae
Published on: January 10, 2018
Anucleate cell production by Escherichia coli delta hns mutant lacking a histone-like protein, H-NS
A Kaidow1, M Wachi, J Nakamura
1Department of Bioengineering, Tokyo Institute of Technology, Yokohama, Japan.
Abstract:
Normal-sized anucleate cells were observed in the cultures of a delta hns mutant strain. Even in nucleate cells, some populations showed irregular intracellular localization of the nucleoids. The delta hns mutant showed reduced ploidy, although initiation of chromosome replication was essentially synchronous as defined by flow cytometric analysis. These results indicate that the delta hns mutant is defective in the mechanisms of chromosome partitioning and chromosome replication.
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