Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Human sperm morphology evaluation pre- and post-Percoll gradient centrifugation

J A Hall1, S B Fishel, J A Timson

  • 1NURTURE (Nottingham University Research & Treatment Unit in Reproduction), Department of Obstetrics and Gynaecology, University Hospital, UK.

Human Reproduction (Oxford, England)
|February 1, 1995
PubMed
Summary

Percoll gradient centrifugation (PGC) improves sperm morphology in patients with moderate teratozoospermia. However, PGC is less effective for severe teratozoospermia, failing to significantly enhance sperm quality in these cases.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Equipping for risk: Lessons learnt from the UK shale-gas experience on assessing environmental risks for the future geoenergy use of the deep subsurface.

The Science of the total environment·2024
Same author

Risk of hospitalisation or death in households with a case of COVID-19 in England: an analysis using the HOSTED data set.

Public health·2022
Same author

Women's experiences of early pregnancy assessment unit services: a qualitative investigation.

BJOG : an international journal of obstetrics and gynaecology·2021
Same author

HOSTED-England's Household Transmission Evaluation Dataset: preliminary findings from a novel passive surveillance system of COVID-19.

International journal of epidemiology·2021
Same author

Limits on the Existence of sub-MeV Sterile Neutrinos from the Decay of ^{7}Be in Superconducting Quantum Sensors.

Physical review letters·2021
Same author

Impacts of operating conditions on efficiency of electrokinetically enhanced filtration for de-watering of orange juice and malt extract.

Food chemistry·2020

Area of Science:

  • Human Reproduction
  • Andrology
  • Sperm Morphology Analysis

Background:

  • Sperm morphology is linked to in vitro fertilization (IVF) potential.
  • Teratozoospermia, characterized by abnormal sperm morphology, affects male fertility.
  • Percoll gradient centrifugation (PGC) is a sperm selection technique.

Purpose of the Study:

  • To evaluate the efficiency of Percoll gradient centrifugation (PGC) in improving sperm morphology.
  • To assess PGC's effectiveness across different severities of teratozoospermia.

Main Methods:

  • Morphological characteristics of spermatozoa from 86 teratozoospermic patients were analyzed.
  • Patients were grouped based on normal morphology percentages (<5%, 5-14%, >14%).
  • Sperm morphology was assessed using Kruger strict criteria after Diff Quik staining.

Related Experiment Videos

Main Results:

  • PGC significantly improved sperm morphology in groups with 5-14% (P < 0.01) and >14% (P < 0.005) normal morphology.
  • PGC did not yield a significant morphological improvement for the group with <5% normal morphology (severe teratozoospermia).
  • PGC effectively selected against head, neck, and immature cell defects, but not tail abnormalities or cytoplasmic fragments.

Conclusions:

  • Percoll gradient centrifugation (PGC) is effective in selecting morphologically superior sperm for patients with moderate to mild teratozoospermia.
  • PGC demonstrates limited efficacy in improving sperm quality for cases of severe teratozoospermia.
  • Specific sperm defects, including head and neck abnormalities, are targeted by PGC, while tail defects are not.