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Related Experiment Videos

Genotypic polymorphisms in experimental metastatic dermal leishmaniasis

R S Pacheco1, J E Martinez, L Valderrama

  • 1Department of Biochemistry and Molecular Biology, Instituto Oswaldo Cruz, FIOCRUZ, Rio de Janeiro, Brazil.

Molecular and Biochemical Parasitology
|February 1, 1995
PubMed
Summary

Genetic analysis of Leishmania (Viannia) guyanensis revealed significant heterogeneity within a single strain. Parasite populations showed diverse kinetoplast DNA (kDNA) restriction patterns, suggesting kDNA sequence polymorphisms can emerge during infection.

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Area of Science:

  • Parasitology
  • Molecular Biology
  • Genetics

Background:

  • Leishmania (Viannia) guyanensis is a significant pathogen responsible for various forms of leishmaniasis.
  • Understanding the genetic diversity within parasite strains is crucial for developing effective control strategies.
  • The strain WHI/BR/78/M5313 was previously characterized as containing both metastatic and non-metastatic populations.

Purpose of the Study:

  • To investigate the genetic heterogeneity and plasticity of the Leishmania (Viannia) guyanensis strain WHI/BR/78/M5313.
  • To determine the relationship between genetic profiles and metastatic potential.
  • To explore the dynamics of genetic variation during host infection.

Main Methods:

  • Molecular karyotyping to assess chromosomal size polymorphisms.

Related Experiment Videos

  • Kinetoplast DNA (kDNA) restriction fragment length polymorphism (RFLP) analysis to identify genetic variations.
  • Cloning and subcloning of parasite populations.
  • In vivo passage in hamsters and recovery from metastatic lesions.

    • Main Results:

    • The strain comprised multiple, closely related parasite populations distinguishable by kDNA restriction patterns (schizodemes).
    • No significant size polymorphisms were detected in the molecular karyotype.
    • Six distinct kDNA minicircle restriction patterns were observed across clones, subclones, and progeny.
    • Metastasis was not associated with any specific schizodeme; all profiles were found in metastatic lesions.
    • Genetic heterogeneity emerged during infection, with kDNA polymorphisms appearing in progeny populations.

    Conclusions:

    • Leishmania (Viannia) guyanensis exhibits considerable genetic heterogeneity and plasticity within a single strain.
    • kDNA restriction analysis is a valuable tool for resolving genetic diversity in Leishmania populations.
    • The emergence of kDNA sequence polymorphisms during infection suggests an adaptive mechanism.
    • Metastatic potential is not predetermined by specific kDNA genotypes within this strain.