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[Bactericidal activity of ciprofloxacin and sparfloxacin. Comparison with others active antibiotics against
C Gastine1, P Y Donnio, M F Travert
1Laboratoire de Bactériologie-Virologie, Faculté de Médecine de Rennes.
Abstract:
Bactericidal activities of ciprofloxacin and sparfloxacin were compared to other antibiotics active against human isolates of Pasteurella multocida. Three human isolates of Pasteurella multocida were used for killing-curve studies with ciprofloxacin and sparfloxacin comparatively to others antibiotics. At 2x the MIC, ciprofloxacin and sparfloxacin exhibited a killing of more than 99.9% of the initial viable cells that was achieved within 6 h of incubation. These activities were faster than those of amoxycillin and cefpodoxime. No regrowth was observed after 24 h of incubation. Doxycycline and clarithromycin used at MICx2 had no bactericidal activities. It was concluded that fluoroquinolones, namely ciprofloxacin and sparfloxacin, can be considered having good bactericidal activity against P. multocida.
Insights
Ciprofloxacin and sparfloxacin demonstrate potent bactericidal activity against Pasteurella multocida, rapidly killing over 99.9% of bacteria within 6 hours. These fluoroquinolones show superior efficacy compared to other antibiotics tested.
Area of Science:
- Microbiology
- Infectious Diseases
- Pharmacology
Background:
- Pasteurella multocida is a significant pathogen causing various infections in humans.
- Understanding antibiotic efficacy against P. multocida is crucial for effective treatment strategies.
Purpose of the Study:
- To compare the bactericidal activities of ciprofloxacin and sparfloxacin against human isolates of Pasteurella multocida.
- To evaluate the speed and extent of bacterial killing by these fluoroquinolones.
Main Methods:
- Killing-curve studies were performed on three human isolates of Pasteurella multocida.
- Antibiotics, including ciprofloxacin, sparfloxacin, amoxycillin, cefpodoxime, doxycycline, and clarithromycin, were tested at concentrations of 2x the Minimum Inhibitory Concentration (MIC).
- Bacterial viability was assessed over a 24-hour incubation period.
Main Results:
- Ciprofloxacin and sparfloxacin achieved a reduction of over 99.9% in viable cells within 6 hours at 2x MIC.
- The bactericidal effect of ciprofloxacin and sparfloxacin was faster than that of amoxycillin and cefpodoxime.
- Doxycycline and clarithromycin showed no significant bactericidal activity at 2x MIC.
- No bacterial regrowth was observed after 24 hours for ciprofloxacin and sparfloxacin.
Conclusions:
- Fluoroquinolones, specifically ciprofloxacin and sparfloxacin, exhibit strong bactericidal activity against human isolates of Pasteurella multocida.
- These antibiotics offer a rapid and effective treatment option for P. multocida infections.