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Beta-adrenoceptor changes in mononuclear leukocytes during pregnancy
1Department of Obstetrics and Gynecology, James H. Quillen College of Medicine, East Tennessee State University, Johnson City 37614, USA.
Summary
Pregnancy alters leukocyte beta 2-adrenoceptor function, decreasing stimulated cyclic adenosine monophosphate (cAMP) production despite unchanged receptor density. This suggests altered signaling pathways during gestation.
Area of Science:
- Immunology
- Pharmacology
- Reproductive Biology
Background:
- Leukocyte beta 2-adrenoceptors share similarities with uterine receptors.
- Pregnancy involves physiological adaptations affecting immune cell function.
Purpose of the Study:
- To investigate changes in beta 2-adrenoceptor density and function during pregnancy.
- To assess cyclic adenosine monophosphate (cAMP) production in leukocytes from pregnant women.
Main Methods:
- Peripheral mononuclear leukocytes were isolated from pregnant and nonpregnant women.
- Beta 2-adrenoceptor density was quantified using 125I-pindolol binding.
- cAMP production was measured via radioimmunoassay under basal and isoproterenol-stimulated conditions.
Main Results:
- A nonsignificant decrease in beta 2-adrenoceptor density was observed during pregnancy.
- Basal cAMP production remained unchanged, but stimulated cAMP production was significantly reduced (44% of control).
- Stimulated cAMP production per receptor was lower in pregnant women, particularly in cells with fewer receptors.
Conclusions:
- Pregnancy significantly alters the coupling of beta 2-adrenoceptors to cAMP production in leukocytes.
- These functional changes occur independently of alterations in receptor density, affinity, or basal cAMP levels.