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Simultaneous purification of biotin-binding proteins-I and -II from chicken egg yolk and their characterization
1Department of Biochemistry, Indian Institute of Science, Bangalore.
The Biochemical Journal
|June 1, 1995
Abstract:
Chicken egg yolk biotin-binding protein-I (BBP-I) has been purified to homogeneity along with the tetrameric BBP-II by a common protocol. The purification includes delipidation of egg yolk by butanol extraction, DEAE-Sephacel chromatography, treatment with guanidinium chloride and biotin-aminohexyl-Sepharose affinity chromatography. The identity of purified BBP-I was ascertained by its physicochemical properties as well as by its immunological cross-reactivity and precursor-product relationship with BBP-II.