Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Whither the anergic B-cell?

D A Fulcher1, A Basten

  • 1Centenary Institute of Cancer Medicine and Cell Biology, Newtown NSW, Australia.

Autoimmunity
|January 1, 1994
PubMed
Summary
This summary is machine-generated.

This study reveals a three-tiered hierarchy of B-cell tolerance in transgenic mice. Different forms of hen egg lysozyme (HEL) induce distinct B-cell responses, affecting B-cell lifespan and leading to either deletion or anergy.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Author reply: To PMID 25041771.

Internal medicine journal·2015
Same author

Treatment outcomes in a cohort of patients with mucosal-predominant pemphigus vulgaris.

Internal medicine journal·2014
Same author

Vancomycin-associated drug reaction with eosinophilia and systemic symptoms syndrome.

Internal medicine journal·2014
Same author

Circulating phenotypic B-1 cells are decreased in common variable immunodeficiency and correlate with immunoglobulin M levels.

Clinical and experimental immunology·2013
Same author

Prevalence of paraproteinaemia in older Australians.

Internal medicine journal·2011
Same author

The role of B cells in transplantation and immunopathic diseases.

Immune network·2010
Same journal

Platycodin D attenuates proliferation, migration, and angiogenesis in RA-stimulated endothelial cells via CD146 modulation.

Autoimmunity·2026
Same journal

CCDC77 and SLC45A3 mediate the genetic mechanism of Hashimoto's thyroiditis through IL-6.

Autoimmunity·2026
Same journal

Multi-cohort transcriptomic analysis with machine learning identifies interferon-related candidate genes in systemic lupus erythematosus.

Autoimmunity·2026
Same journal

IgA Vasculitis with necrotizing arteritis: a multicenter retrospective study from the French Vasculitis Study Group and systematic review of the literature.

Autoimmunity·2026
Same journal

Global research trends in ferroptosis in autoimmune diseases: a bibliometric and visual analysis (2018-2025).

Autoimmunity·2026
Same journal

Monocyte exhaustion associated with systemic lupus erythematosus.

Autoimmunity·2026
See all related articles

Area of Science:

  • Immunology
  • Transgenic models
  • Self-tolerance

Background:

  • Investigating the kinetics of potentially autoreactive B-cells is crucial for understanding self-tolerance.
  • Transgenic models offer a controlled environment to study immune responses.

Purpose of the Study:

  • To investigate the kinetics of potentially autoreactive B-cells in a double-transgenic model of self-tolerance.
  • To characterize the hierarchy of B-cell unresponsiveness based on B-cell lifespan.

Main Methods:

  • Created two types of transgenic mice: one expressing a high-affinity receptor for hen egg lysozyme (HEL), and another expressing HEL in soluble (sHEL) or membrane-bound (mHEL) forms.
  • Produced double-transgenic (Dbl-Tg) mice by mating or bone marrow transfer.
  • Measured B-cell lifespan using 5-bromo-2'-deoxyuridine (BrdU) labeling.

Related Experiment Videos

Main Results:

  • Exposure to membrane-bound HEL (mHEL) rapidly deleted immature B-cells with a median lifespan of ~15 hours.
  • B-cells exposed to soluble HEL (sHEL) above a threshold became anergic in the spleen and died within three days.
  • B-cells exposed to sHEL below the threshold remained "indifferent" with a normal lifespan of 4-5 weeks.

Conclusions:

  • A three-tiered hierarchy of B-cell unresponsiveness exists, determined by the form and concentration of the autoantigen.
  • Immature B-cells are sensitive to mHEL-induced deletion, while mature B-cells can become anergic to sHEL.
  • These findings provide insights into mechanisms of peripheral self-tolerance.