Use of confocal laser scanning microscopy in radioautographic study

Confocal laser scanning microscopy was used to visualize the radioautograph at the light-microscope level. Confocal reflectance images from developed silver grains were overlaid with the differential interference contrast image of the tissue. With this technique, a well-focused radioautograph can be taken of both tissue sections and all of the silver grains dispersed in the thick emulsion layer. Further application of this microscopy is discussed.