Ultrastructural and radioautographic studies on DNA synthesis in Leydig cells on aging mouse testis

A morphological observation and a radioautographic study were carried out to detect the ultrastructure and aging changes of DNA synthesis of the Leydig cells, whose structure has been observed and the area of the lipid droplets in the cytoplasm calculated. The observation by electron microscopy showed that Leydig cells contained abundant mitochondria and endoplasmic reticulum and that the area of the lipids in the cytoplasm changed with aging. Only little lipid droplets were found in embryonic and early postnatal stages, but they increased from postnatal two weeks onwards and no significant differences were found from 9 months to 21 months. The DNA synthesis in these cells was not vigorous at embryonic day 19, explored by light microscopic radioautography 1 hr. following a single injection of 3H-thymidine. The labeling index increased slightly before six months of age and reached its peak at 9 months. There was no significant difference found in the number of labelled cells from that time on. A relatively high level was maintained at adult and senescent periods. Our results demonstrate that the generation of the Leydig cells contained plentiful lipid droplets was vigorous at adult and senescence.