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Related Experiment Videos

Leukemia-specific allogeneic donor T cells: quantification by limiting dilution assay

M Salomo1, J Steinmann, B Glass

  • 1Institute of Immunology, University of Kiel, Germany.

Bone Marrow Transplantation
|February 1, 1995
PubMed
Summary

Allogeneic T cells can target leukemia cells. Researchers found significant inter-individual differences in leukemia-reactive T cell potential, identifying promising candidates for leukemia immunotherapy.

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Area of Science:

  • Immunology
  • Hematology
  • Oncology

Background:

  • Allogeneic T cells show potential in discriminating between leukemia and normal hematopoietic cells.
  • Previous studies suggest T cell-mediated graft-versus-leukemia (GVL) effects.

Purpose of the Study:

  • To analyze the frequency and specificity of leukemia-reactive T cells in syngeneic and allogeneic donors.
  • To evaluate the potential of these T cells for leukemia immunotherapy.

Main Methods:

  • Limiting dilution assays were used to quantify interleukin-2-producing (TH1) and cytotoxic (CTLp) T cells.
  • Leukemia blasts from a common acute lymphoblastic leukemia (ALL) patient and EBV-transformed cells were used as targets.
  • Effector cells were derived from the patient, his syngeneic brother, and HLA-mismatched allogeneic donors.

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Main Results:

  • Neither the patient nor his brother generated anti-leukemic T cells.
  • Allogeneic donors exhibited a wide range of anti-leukemic CTLp frequencies (0-68 million).
  • Anti-leukemic TH1 cells were not detected in any donor group.

Conclusions:

  • Significant inter-individual variability exists in the GVL potential of allogeneic T lymphocytes.
  • T cell lines reactive against leukemia but not normal cells can be identified.
  • These specific T cell lines represent potential candidates for human leukemia immunotherapy.