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IS292: a novel insertion element from Agrobacterium

C Ponsonnet1, P Normand, G Pilate

  • 1Laboratoire d'Ecologie Microbienne du Sol, Université Claude Bernard Lyon 1, Villeurbanne, France.

Microbiology (Reading, England)
|April 1, 1995
PubMed
Summary
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Researchers identified a novel insertion sequence, IS292, in Agrobacterium. This sequence shares similarities with elements in Rhizobium, suggesting potential horizontal gene transfer in plant-associated bacteria.

Area of Science:

  • Microbiology
  • Molecular Biology
  • Genetics

Background:

  • Agrobacterium species are significant plant pathogens.
  • Insertion sequences (IS) are mobile genetic elements that can influence bacterial genomes.
  • Understanding IS elements in Agrobacterium is crucial for studying bacterial evolution and pathogenicity.

Purpose of the Study:

  • To identify and characterize a novel insertion sequence element in a nopaline-type Agrobacterium strain.
  • To investigate the relationship of this new element with known insertion sequences in other bacteria.
  • To determine the prevalence of this insertion sequence in various Agrobacterium isolates.

Main Methods:

  • DNA sequencing of the novel insertion sequence (IS292).
  • Bioinformatic analysis to compare IS292 with known insertion sequences.

Related Experiment Videos

  • DNA hybridization techniques to detect IS292-like sequences in other Agrobacterium isolates.

    • Main Results:

    • A new insertion sequence, IS292, of 2494 bp was identified in an Agrobacterium strain from poplar.
    • IS292 possesses 21 bp inverted terminal repeats and generates 10 bp direct repeats upon integration.
    • IS292 showed no similarity to other Agrobacterium IS elements but was similar to ISR/1 from Rhizobium leguminosarum bv. viciae.
    • IS292-like sequences were detected in various Agrobacterium isolates, including different strains from the same biotope.

    Conclusions:

    • IS292 represents a novel insertion sequence element in Agrobacterium.
    • The similarity to Rhizobium IS elements suggests potential horizontal gene transfer events.
    • The widespread occurrence of IS292-like sequences indicates their mobility and potential role in Agrobacterium populations.