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Morphology and morphogenesis of hepatitis E virus (strain 87A)
Chinese Medical Journal
|February 1, 1995
Summary
Hepatitis E virus (HEV) strain 87A replication occurs in the cytoplasm, associated with the endoplasmic reticulum. This RNA virus, approximately 30 nm in diameter, exhibits unique morphology distinct from caliciviruses.
Area of Science:
- Virology
- Cell Biology
- Microbiology
Background:
- Hepatitis E virus (HEV) is a significant cause of viral hepatitis worldwide.
- Understanding HEV morphology and replication is crucial for developing antiviral strategies.
Purpose of the Study:
- To elucidate the morphology and morphogenesis of Hepatitis E virus (HEV) strain 87A.
- To investigate the cellular sites of HEV replication and assembly.
Main Methods:
- Electron Microscopy (EM)
- Immune Electron Microscopy (IEM)
- Observation of infected cell cultures
Main Results:
- HEV replication and assembly are associated with the rough endoplasmic reticulum, inclusion bodies, and microfibrils within the cytoplasm.
- Nuclear alterations including chromatin condensation and nucleolar dispersion were observed.
- HEV strain 87A virions are approximately 30 nm, roughly spherical, with irregular outlines and occasional surface spikes, distinguishing them from caliciviruses.
Conclusions:
- HEV strain 87A is confirmed as an RNA virus.
- The findings suggest HEV replication and assembly occur in close association with cellular organelles.
- A new genus, heparnavirus, within the Caliciviridae family should be proposed for HEV.