[The study of repairing ability of freeze-dried bone allograft]

There were many methods to preserve allogeneic bone allograft, however, a lot of studies have shown that the freeze-dried bone allograft banking is the best one. But it is not clear what temperature to lyophilize the allograft is better. In this experiment, we used method, in which the temperature of lyophilization was raising naturally and regularly, to lyophilize the allograft. The repairing ability of the freeze-dried allograft was studied in rabbits and comparison was made with that of autograft and fresh allograft. The results indicated that the repairing ability of bone defects was not significantly different between autograft and freeze-dried allograft. There was no significant difference between autograft and fresh allograft 3 weeks after transplantation, but significant difference was noted 8 weeks after grafting. It is suggested that this might be associated with the latent period before immunological rejection and the method itself, in which the temperature of lyophilization raising naturally is beneficial.