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Synthesis and separation of protected tripeptide epimers by RP-HPLC
1Department of Organic Chemistry, Martin Luther University Halle-Wittenberg, Germany.
International Journal of Peptide and Protein Research
|March 1, 1995
Abstract:
Twenty three epimeric pairs of protected tripeptides of the type Z-Ala-Xaa-Val-OMe have been synthesized by conventional coupling procedures in solution. Optimal conditions for baseline separation of LLL/LDL epimers were found by high-performance liquid chromatography on a RP-18 column with aqueous methanol as mobile phase. The hydrophobic parameters (log kw) were determined for all tripeptides.