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Related Experiment Videos

Structural interaction between the nuclear pore complex and a specific translocating RNP particle

H Mehlin1, B Daneholt, U Skoglund

  • 1Department of Cell and Molecular Biology, Medical Nobel Institute, Karolinska Institutet, Stockholm, Sweden.

The Journal of Cell Biology
|June 1, 1995
PubMed
Summary
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Balbiani ring (BR) premessenger RNP particles interact with the nuclear pore complex (NPC) border during translocation. This interaction involves rotation, ensuring ordered movement through the NPC central channel.

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Structural Biology

Background:

  • Balbiani ring (BR) premessenger ribonucleoprotein (RNP) particles are large structures involved in gene expression in dipteran salivary glands.
  • The transport of these particles through the nuclear pore complex (NPC) is a critical step in gene regulation.
  • Previous studies visualized BR RNP particles during nuclear pore passage but lacked detailed interaction analysis.

Purpose of the Study:

  • To investigate the interactions between BR RNP particles and the nuclear pore complex (NPC) periphery during translocation.
  • To elucidate the role of these interactions in the ordered passage of BR RNP particles through the NPC.

Main Methods:

  • Electron microscopy was used to observe BR RNP particle transport in Chironomus tentans larval salivary gland cells.

Related Experiment Videos

  • Electron microscope tomography was employed to study the detailed connections between RNP particles and the NPC border.
  • Analysis focused on different stages of particle translocation through the NPC.

    • Main Results:

    • BR RNP particles adopt a ring-like structure that straightens upon entering the NPC central channel, with the 5' end leading.
    • The 3' terminal domain of the RNP ribbon consistently contacts the NPC's nuclear ring, with variable contact points.
    • Observed particle-nuclear ring interactions correlate with translocation stage, suggesting a rotation of the RNP ring.

    Conclusions:

    • The interactions between the BR RNP particle and the nuclear ring facilitate ordered translocation through the NPC.
    • A rotation of the RNP ring occurs secondary to its passage through the NPC central channel.
    • Initial contact between the RNP's 5' end and the NPC entrance is crucial for successful premessenger RNP particle translocation.