Shelf-life determination of prostaglandin E1 injections

A rapid stability indicating assay for the determination of prostaglandin E1 (PGE1) in pharmaceuticals was developed using High Pressure Liquid Chromatography (HPLC). A solution of 20 micrograms/ml PGE1 in 4% alcohol and 0.9% saline has an activation energy of 18,683 cal/mol and the predicted shelf-lives were: at 4 degrees C t95 = 51.8 days and t90 = 106.5 days and at 25 degrees C t95 = 4.8 days and t90 = 9.8 days.