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The cytochemical profile of visceral mesothelium under the influence of lactated-hyperosmolar peritoneal dialysis
L Gotloib1, A Shostak, V Wajsbrot
1Department of Nephrology, Central Emek Hospital, Afula, Israel.
Abstract:
Male albino mice had one daily intraperitoneal injection of 4.25 g/100 ml glucose concentration fluid for peritoneal dialysis at pH 5.0-5.2, for a period of 30 days. At the end of the experimental periods, mesothelial cell imprints were taken from the peritoneal layer of the anterior liver surface. Histochemical staining of imprints obtained from mice exposed to the peritoneal dialysis fluid showed a consistently increased activity of: (a) enzymes associated with the cell membrane: Na-K-ATP-ase, alkaline phosphatase and 5-nucleotidase; (b) cytoplasmic enzymes: acid phosphatase and cytochrome oxidase, and (c) a modestly increased activity of glucose-6-phosphatase. These changes, which are not far from those observed in activated mesothelial cells, suggest that exposure of mesothelial cells to high glucose concentrations of PD fluid is associated with increased production and disposal of energy to be used for maintaining the constancy of the cellular environment and, probably, for fuelling the transcellular transport of solutes of large molecular size.
Insights
Peritoneal dialysis fluid with high glucose concentrations increases enzyme activity in mouse mesothelial cells. This suggests enhanced cellular energy production and transport functions in response to dialysis fluid exposure.
Area of Science:
- Biochemistry
- Cell Biology
- Nephrology
Background:
- Peritoneal dialysis (PD) is a crucial treatment for kidney failure.
- Mesothelial cells line the peritoneal cavity and are exposed to PD fluid.
- Understanding mesothelial cell response to PD fluid is vital for optimizing treatment.
Purpose of the Study:
- To investigate the impact of high glucose concentration PD fluid on mesothelial cell enzyme activity.
- To elucidate the metabolic and functional adaptations of mesothelial cells to PD fluid exposure.
Main Methods:
- Male albino mice received daily intraperitoneal injections of high glucose PD fluid for 30 days.
- Mesothelial cell imprints were collected from the liver surface.
- Histochemical staining was used to assess enzyme activity.
Main Results:
- Significantly increased activity of cell membrane enzymes (Na-K-ATP-ase, alkaline phosphatase, 5-nucleotidase) was observed.
- Increased activity of cytoplasmic enzymes (acid phosphatase, cytochrome oxidase) was noted.
- A modest increase in glucose-6-phosphatase activity was detected.
Conclusions:
- High glucose PD fluid stimulates mesothelial cells, leading to heightened enzyme activity.
- These changes indicate increased energy production and potential enhancement of transcellular transport.
- Mesothelial cells adapt metabolically to the high glucose environment of PD fluid.