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Isolation of Murine Peritoneal Macrophages to Carry Out Gene Expression Analysis Upon Toll-like Receptors Stimulation
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Molecular cloning and posttranscriptional regulation of macrophage inflammatory protein-1 alpha in alveolar

M M Shi1, J J Godleski, J D Paulauskis

  • 1Department of Environmental Health, Harvard School of Public Health, Boston, MA 02115, USA.

Biochemical and Biophysical Research Communications
|June 6, 1995
PubMed

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The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...