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Improved outcome of rat lung transplantation with modification of the nonsuture external cuff technique
Background:
The development of lung transplantation in inbred rats has simplified immunologic studies concerning lung allografts. Unfortunately, up to now, such models have been limited by the high level of surgical expertise required.
Methods:
In this study 92 rats underwent left lung transplantation with a simplified cuff technique. The feasibility and reliability of this technique was determined by examining graft patency through the use of gross and histologic examination, angiography, and bronchography.
Results:
On gross examination, grafts looked well perfused and ventilated. Histologic examination showed little evidence of foreign body reaction against the Teflon cuff devices, and angiography showed no abnormalities; however, bronchography did show signs of airway stricture at the site of the anastomosis.
Conclusion:
We conclude that the nonsuture external cuff technique with Teflon devices represents a reliable, safe, and fast method for performing orthotopic left lung transplantation in the rat.