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Related Experiment Videos

Intracytoplasmic sperm injection in the mouse

Y Kimura1, R Yanagimachi

  • 1Department of Anatomy and Reproductive Biology, University of Hawaii School of Medicine, Honolulu 96822, USA.

Biology of Reproduction
|April 1, 1995
PubMed
Summary

Piezo-driven micropipettes improve intracytoplasmic sperm injection (ICSI) success in mice. This advanced technique resulted in viable blastocysts and healthy offspring, enhancing understanding of fertilization and ICSI procedures.

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Area of Science:

  • Reproductive Biology
  • Developmental Biology
  • Assisted Reproductive Technology

Background:

  • Intracytoplasmic sperm injection (ICSI) is a key assisted reproductive technology.
  • Conventional mechanical pipettes for ICSI can cause oocyte damage.
  • Improving ICSI techniques is crucial for reproductive success.

Purpose of the Study:

  • To evaluate the efficacy of piezo-driven micropipettes in mouse ICSI.
  • To compare the success rates of piezo-driven versus conventional ICSI.
  • To assess the developmental potential of embryos created via piezo-ICSI.

Main Methods:

  • Intracytoplasmic sperm injection using a piezo-driven micropipette in mice.
  • Assessment of oocyte survival and blastocyst development in vitro.
  • Embryo transfer to foster mothers and evaluation of full-term development.

Main Results:

  • High oocyte survival rate (80%) after piezo-driven ICSI.
  • Significant blastocyst development rate (approx. 70%) in vitro.
  • Successful term development (30%) and normal growth of pups to adulthood.

Conclusions:

  • Piezo-driven micropipettes offer a more effective method for mouse ICSI.
  • This technique improves embryo quality and developmental outcomes.
  • Further research can elucidate the mechanisms of fertilization and ICSI.

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