Studies on the condition of fermentation of pullulan by Aureobasidium pullulans

Studies on optimal conditions for pullulan fermentation based on the results obtained from a shaking flask were carried out in a 16-L auto-controlling fermentor. It found that the optimal DE value of starch hydrolyzate was 40-50 when 10% starch hydrolysate was used as carbon source. The optimal concentration of ammonium sulfate in the medium for fermentation was different from that of the shaking flask. The fermentation kinetics and effects of seed age, seed volume, airflow rate, pressure of tank, agitation speed and number of vane group on the production of pullulan were investigated.