Green fluorescent protein. The green revolution

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Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli
08:46

Green Fluorescent Protein-based Expression Screening of Membrane Proteins in Escherichia coli

Published on: January 6, 2015

T Stearns1

  • 1Department of Biological Sciences, Stanford University, California 94305-5020, USA.

Current Biology : CB
|March 1, 1995
PubMed

Abstract:

Green fluorescent protein allows gene expression and protein localization to be observed in living cells.

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Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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