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Updated: May 9, 2026

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Initiation of V(D)J recombination in a cell-free system

D C van Gent1, J F McBlane, D A Ramsden

  • 1Laboratory of Molecular Biology, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.

Cell
|June 16, 1995
PubMed
Summary

Nuclear extracts from pre-B cells cleave DNA at specific sites during V(D)J recombination. Recombinant RAG1 and RAG2 proteins are key components of this DNA cutting process, essential for immune system development.

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