The effect of opiates on the secretion of transmitter from amphibian motor nerve terminals

The effects of dynorphin-A, dermorphine and morphine on the secretion of transmitter from the toad (Bufo marinus) motor nerve terminal have been determined. Intracellular recordings of miniature end plate potentials (m.e.p.p.s) and evoked end plate potentials (e.p.p.s) were used to estimate quantal content (m) and binomial parameters p and n. Dynorphin-A, and to a lesser extent morphine, decreased (m) while dermorphine had no significant effect on m. Dynorphin-A (ED50 = 24 microM) was 21 times more potent then morphine (ED50 = 510 microM) in decreasing m. The decrease in m produced by dynorphin-A and morphine was accompanied by a greater decrease in the variance (S2) of number of quanta secreted per stimulation over the recording period. The decrease in m produced by dynorphin-A, and to a lesser extent by morphine, is probably mediated by the opiates acting on kappa-opioid receptors.