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Published on: October 21, 2010
Evaluation of a broth microdilution antifungal susceptibility test with a pH indicator: comparison with the broth
C Fournier1, A Gaspar, F Boillot
1Laboratoire de Mycologie Fondamentale et Appliquée aux Biotechnologies Industrielles, Faculté de Pharmacie, Lyon, France.
Abstract:
A broth microdilution antifungal susceptibility test with a pH indicator (bromocresol purple) in a synthetic medium was evaluated. This method measures cellular activity instead of simply a change in biomass. The variations of pH caused by fungal activity were measured by changes in optical densities at 450 nm. The MICs50 obtained were compared with the MICs found by the classical broth macrodilution procedure. In most cases broth micro- and macrodilution MICs were in agreement for amphotericin B, fluconazole, flucytosine and nystatin.
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