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Updated: Feb 28, 2026

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Cortisol Measurement in Koala Phascolarctos cinereus Fur
Published on: August 23, 2019
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An immuno-enzymatic assay of cortisol using E. coli beta-galactosidase as label
Journal of Immunological Methods
|March 1, 1976
Abstract:
An enzyme-assay of cortisol was established by using E. coli beta-galactosidase as a marker. The number of cortisol residues per molecule of enzyme and the method of separation of free from antibody-bound enzyme proved to be critical factors for the assessment of the assay. A sensitivity ranging from 100 to 150 pg of cortisol per tube has been achieved. A comparsion with other methods, i.e. competitive protein binding assay, radioimmunoassay and fluorimetry, is reported.

