Affinity chromatography. A new technique for partial purification of human leucocyte migration inhibitory factor

Human leucocyte migration inhibitory factor (LIF) was subjected to affinity column chromatography on Sepharose-bound alpha-L-fucose. Following the addition of alpha-L-fucose to the running buffer adsorbed lymphokine was eluted almost quantitatively. A minimum of 70-fold purification of LIF was achieved.