Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Improved techniques for kidney transplantation in mice

Z Zhang1, C Schlachta, J Duff

  • 1Department of Surgery and Medicine, University of Western Ontario, London, Canada.

Microsurgery
|January 1, 1995
PubMed
Summary

Researchers developed improved microsurgical techniques for mouse kidney transplantation, achieving up to 90% success. This reliable model aids in studying kidney transplant rejection at the molecular level.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adherence to Protocol Recommendations for Children With Wilms Tumour in Two Consecutive Studies in the United Kingdom and Ireland-Does Variation Matter?

Pediatric blood & cancer·2026
Same author

Sex differences in survival from melanoma of the skin: The role of age, anatomic location and stage at diagnosis: A CONCORD-3 study in 59 countries.

European journal of cancer (Oxford, England : 1990)·2025
Same author

Abstracts of presentations to the Annual Meetings of the Canadian Society of Colon and Rectal Surgeons Canadian Association of General Surgeons Canadian Association of Thoracic Surgeons: Canadian Surgery Forum, London, Ont., Sept. 19 to 22, 2002.

Canadian journal of surgery. Journal canadien de chirurgie·2023
Same author

Canadian Surgery Forum 2018: St. John's, NL Sept. 13-15, 2018.

Canadian journal of surgery. Journal canadien de chirurgie·2022
Same author

Canadian Surgery Forum.

Canadian journal of surgery. Journal canadien de chirurgie·2022
Same author

2021 Canadian Surgery Forum: Virtual, online Sept. 21-24, 2021.

Canadian journal of surgery. Journal canadien de chirurgie·2022

Area of Science:

  • Surgical Innovation
  • Transplantation Immunology
  • Small Animal Models

Background:

  • Kidney transplantation is a critical treatment for end-stage renal disease.
  • Developing reliable animal models is essential for studying transplantation immunology.
  • Existing mouse models for kidney transplantation may have limitations in reproducibility or success rates.

Purpose of the Study:

  • To describe improved microsurgical techniques for left renal transplantation in mice.
  • To establish a stable and reproducible mouse model for kidney transplantation research.
  • To facilitate the study of kidney allograft rejection mechanisms at the molecular level.

Main Methods:

  • Left renal transplantation using end-to-side anastomoses for renal vein and aorta.

Related Experiment Videos

  • Donor renal vein anastomosed to the inferior vena cava.
  • Donor aortic cuff anastomosed to the recipient aorta.
  • Urinary tract reconstruction by suturing the donor ureter with a bladder patch to the recipient bladder.

    • Main Results:

    • The described microsurgical techniques achieve high success rates, up to 90%.
    • The model is stable and reproducible, suitable for experimental studies.
    • The technique allows for detailed investigation of immunological rejection processes.

    Conclusions:

    • The improved microsurgical techniques provide a robust and effective method for mouse kidney transplantation.
    • This model is a valuable tool for advancing the understanding of kidney allograft rejection.
    • Further research into molecular mechanisms of rejection can be effectively conducted using this model.