[The production and standardization of streptolysin-O]

The production of streptolysin-O by using the S84 strain of beta hemolytic streptococcus is explained. The Rantz-Randall and Hartley media with the Gooder method have been used because better results could be obtained in a shorter time and it was more economical. In obtaining a potent streptolysin-O not only the method but the strains and media must be taken into consideration. The hemolytic potency of the obtained streptolysin-O is within the necessary limits and there is no need to concentrate it. The pH of the media was adjusted 5-6 hours after the culture began because the formed acid during the growth of streptococci decreased the growth of the organism and its toxin yield. The toxin was stored in the deepfreeze for daily use. In ASO tests it was subjected to a reduction process with sistein-L for 10-30 minutes.