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A bisulfite method of 5-methylcytosine mapping that minimizes template degradation
A M Raizis1, F Schmitt, J P Jost
1Friedrich Miescher Institut, Basel, Switzerland.
Analytical Biochemistry
|March 20, 1995
Abstract:
The bisulfite method is a highly sensitive approach to 5-methylcytosine mapping that utilizes the capability of the polymerase chain reaction to exponentially amplify DNA. We have observed that the bisulfite reaction results in a significant level of template degradation due to DNA depurination. Furthermore, our data suggest that the DNA fragmentation which occurs limits the sensitivity of the method. We describe a simple solution to limit degradation of the DNA template.