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Related Experiment Videos

D-dimer tests detect both plasmin and neutrophil elastase derived split products

R Seitz1, L Lerch, A Immel

  • 1Department of Haematology/Oncology, Philipps University Hospitals, Marburg, Germany.

Annals of Clinical Biochemistry
|March 1, 1995
PubMed
Summary

Neutrophil elastase can increase D-dimer levels, a marker of blood clots. This study suggests neutrophil elastase influences D-dimer in inflammatory conditions, impacting diagnosis.

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Area of Science:

  • Biochemistry
  • Hematology
  • Immunology

Background:

  • Plasmin and neutrophil elastase degrade fibrin(ogen).
  • Elevated D-dimer levels in inflammatory diseases may involve elastase.
  • Investigating the role of neutrophil elastase in D-dimer formation is crucial.

Purpose of the Study:

  • To investigate the potential role of neutrophil elastase in elevating D-dimer levels.
  • To correlate D-dimer values with markers of coagulation and neutrophil activation in inflammatory bowel disease patients.

Main Methods:

  • In vitro incubation of fibrinogen and fibrin clots with elastase.
  • Measurement of D-dimer immunoreactivity using commercial ELISA kits.
  • Analysis of plasma D-dimer levels and elastase-alpha 1-antitrypsin complexes in inflammatory bowel disease patients.

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Main Results:

  • In vitro elastase treatment significantly increased D-dimer immunoreactivity.
  • Plasma D-dimer levels in inflammatory bowel disease patients correlated with thrombin and plasmin activation markers.
  • D-dimer levels also showed a significant correlation with elastase-alpha 1-antitrypsin complexes.

Conclusions:

  • Neutrophil elastase contributes to elevated D-dimer levels in inflammatory disorders.
  • D-dimer levels in inflammatory conditions are influenced by neutrophil elastase activity.
  • Developing tests to differentiate haemostasis activation from neutrophil proteolysis could aid clinical management.