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Related Experiment Videos

Fluorescence lifetime-based sensing in tissues: a computational study

C L Hutchinson1, J R Lakowicz, E M Sevick-Muraca

  • 1Purdue University, School of Chemical Engineering, West Lafayette, Indiana 47907, USA.

Biophysical Journal
|April 1, 1995
PubMed
Summary
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ADVANCES IN FLUORESCENCE SPECTROSCOPY: MULTI-PHOTON EXCITATION, ENGINEERED PROTEINS, MODULATION SENSING AND MICROSECOND RHENIUM METAL-LIGAND COMPLEXES.

Acta physica Polonica: A·2019

We numerically simulated light propagation in tissue to measure fluorescent lifetimes. Frequency-domain measurements can quantify fluorescent lifetimes (tau) noninvasively, independent of tissue optical properties.

Area of Science:

  • Biomedical Optics
  • Photonic Imaging
  • Biophysical Measurements

Background:

  • Accurate measurement of fluorescent lifetimes in biological tissues is crucial for diagnostic applications.
  • Tissue optical properties, such as absorption and scattering, can complicate fluorescence measurements.
  • Noninvasive techniques are highly desirable for in vivo monitoring.

Purpose of the Study:

  • To investigate the feasibility of using photon diffusion equation to predict light distribution in a uniform fluorophore tissue model.
  • To explore the potential of time-dependent and frequency-domain measurements for quantifying fluorescent lifetimes.
  • To assess the independence of fluorescent lifetime quantitation from local absorption and scattering variations.

Main Methods:

  • Numerical solution of the photon diffusion equation.

Related Experiment Videos

  • Modeling light propagation in a tissue system with uniform fluorophore concentration.
  • Analysis of time-dependent and frequency-domain measurements (phase-shift, theta).

    • Main Results:

    • Time-dependent light propagation measurements can monitor fluorescent lifetimes in tissues.
    • Frequency-domain measurements of phase-shift (theta) can quantify fluorescent lifetimes (tau).
    • Quantitation of fluorescent lifetimes is achievable independent of local absorption and scattering properties.

    Conclusions:

    • Noninvasive diagnostic monitoring is possible through fluorescent lifetime quantitation.
    • This approach is particularly effective when fluorophore lifetime is comparable to photon migration times.
    • Frequency-domain measurements offer a promising method for accurate in vivo fluorescence lifetime imaging.