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A Protocol for Phage Display and Affinity Selection Using Recombinant Protein Baits
Published on: February 16, 2014
Phage display of catalytically active staphylococcal nuclease
1Department of Chemistry, University of California, Berkeley 94720, USA.
Bioorganic & Medicinal Chemistry
|December 1, 1994
Abstract:
Staphylococcal nuclease (SNase), a 14 kD enzyme that catalyzes the hydrolysis of single- and double-stranded nucleic acid, was fused to the N-terminus of the gene III (pIII) protein of filamentous phage fdtet. The SNase-pIII protein is infective and the catalyzes DNA hydrolysis, demonstrating that functional SNase can be displayed on the phage surface.
