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Related Experiment Videos

Wingless induces transdetermination in developing Drosophila imaginal discs

L Maves1, G Schubiger

  • 1Department of Zoology NJ-15, University of Washington, Seattle 98195, USA.

Development (Cambridge, England)
|May 1, 1995
PubMed
Summary

Ectopic expression of the Drosophila gene wingless induces transdetermination of foreleg imaginal disc cells to wing cells. This discovery offers a new molecular approach to understanding cell determination changes in development.

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Area of Science:

  • Developmental Biology
  • Genetics
  • Cell Biology

Background:

  • Drosophila imaginal discs are crucial for adult appendage development and studying cell determination.
  • Transdetermination, the switching of determined cell fates, is a key phenomenon in imaginal discs.
  • Understanding the molecular basis of transdetermination provides insights into maintaining cell determination.

Purpose of the Study:

  • To investigate the molecular mechanisms underlying transdetermination in Drosophila imaginal discs.
  • To identify genes that can induce transdetermination in vivo.
  • To explore the relationship between gene expression and cell fate switching.

Main Methods:

  • Ectopic expression of the Drosophila gene wingless in foreleg imaginal discs.

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  • Observation of transdetermination events in situ without disc fragmentation.
  • Localization of transdetermining cells within the foreleg disc.

    • Main Results:

    • Ectopic wingless expression successfully induced transdetermination of foreleg imaginal disc cells into wing cells.
    • Transdetermination occurred in intact larval discs, not requiring prior fragmentation.
    • The affected cells were localized to the dorsal region of the foreleg disc.
    • The wingless-induced transdetermination closely mimicked natural leg-to-wing switches observed after disc culture.

    Conclusions:

    • The Drosophila gene wingless is capable of inducing transdetermination in imaginal discs.
    • This provides a novel in vivo system for molecularly dissecting transdetermination.
    • The findings shed light on the molecular requirements for maintaining and switching cell determination states.