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Related Experiment Videos

CSF-1 and mouse preimplantation development in vitro

P Bhatnagar1, V E Papaioannou, J D Biggers

  • 1Department of Cell Biology, Harvard Medical School, Boston, MA 02115, USA.

Development (Cambridge, England)
|May 1, 1995
PubMed
Summary
This summary is machine-generated.

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Macrophage colony stimulating factor (M-CSF) enhances early mouse embryo development in vitro. M-CSF accelerates blastocyst formation and increases trophoblast cell numbers, supporting normal fetal development.

Area of Science:

  • Developmental biology
  • Cell biology
  • Reproductive science

Background:

  • Early embryonic development is crucial for successful reproduction.
  • In vitro culture media support preimplantation development.
  • Growth factors can influence embryonic cell differentiation.

Purpose of the Study:

  • To investigate the effects of macrophage colony stimulating factor (M-CSF) on mouse zygote to blastocyst development.
  • To determine the optimal concentration and timing of M-CSF exposure.
  • To assess the impact of M-CSF on embryonic cell numbers, specifically trophoblast and inner cell mass.

Main Methods:

  • Mouse embryos (zygote to blastocyst stage) cultured in KSOM medium.
  • Addition of varying concentrations of M-CSF to the culture medium.

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  • Assessment of blastocyst cavity formation, cell numbers (total, trophoblast, inner cell mass) at specific time points (day 4 and 5).

    • Main Results:

    • M-CSF significantly accelerated blastocyst cavity formation by day 4.
    • M-CSF increased overall embryonic cell number, primarily due to a ~30% rise in trophoblast cells by day 5.
    • No significant effect on inner cell mass cell number was observed.
    • Optimal M-CSF concentration was around 540 U/ml.
    • M-CSF effects were observed even with partial exposure during culture.

    Conclusions:

    • M-CSF promotes in vitro preimplantation development in mice.
    • M-CSF specifically enhances trophoblast cell proliferation.
    • M-CSF treatment is compatible with subsequent normal in vivo fetal development.