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Related Experiment Videos

Mutations affecting donor preference during mating type interconversion in Saccharomyces cerevisiae

K S Weiler1, L Szeto, J R Broach

  • 1Department of Molecular Biology, Princeton University, New Jersey 08544-1014, USA.

Genetics
|April 1, 1995
PubMed
Summary
This summary is machine-generated.

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The CHL1 gene in yeast is crucial for mating type switching, ensuring the correct genetic information is selected. Mutations in CHL1 disrupt this process, leading to random donor selection during mating type interconversion.

Area of Science:

  • Molecular Biology
  • Yeast Genetics
  • Cellular Regulation

Background:

  • Homothallic Saccharomyces cerevisiae strains switch mating types (a to alpha or alpha to a) via gene conversion at the MAT locus.
  • Donor preference, where MATa cells typically use HML and MAT alpha cells use HMR, regulates this switching process.

Purpose of the Study:

  • To identify genetic factors regulating donor preference during yeast mating type interconversion.
  • To investigate the role of CHL1 in the mechanism of donor selection.

Main Methods:

  • Isolation and characterization of yeast mutants exhibiting altered donor preference.
  • Analysis of chromosome rearrangements and mutations affecting mating type switching.
  • Phenotypic analysis of CHL1 null mutants in mating type interconversion.

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Main Results:

  • Mutants with altered donor preference included those with chromosome rearrangements or mutations at MAT/HML.
  • A recessive allele of CHL1 (required for chromosome segregation) was identified in a mutant with impaired donor preference.
  • CHL1 null mutants showed random donor locus selection in MATa cells but normal preference in MAT alpha cells.

Conclusions:

  • Chl1 protein (Chl1p) plays a significant role in regulating donor preference during yeast mating type switching.
  • The findings support a model where Chl1p contributes to an intrinsic bias in donor selection, particularly in MATa cells.