Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Genes that regulate both development and longevity in Caenorhabditis elegans

P L Larsen1, P S Albert, D L Riddle

  • 1Molecular Biology Program, University of Missouri, Columbia 65211, USA.

Genetics
|April 1, 1995
PubMed
Summary

Genetic analysis in Caenorhabditis elegans reveals key pathways controlling dauer larva formation and adult lifespan. Specific gene mutations significantly extend lifespan, offering insights into aging mechanisms.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cancer mortality and geographic inequalities: a detailed descriptive and spatial analysis of social determinants across US counties, 2018-2021.

Public health·2024
Same author

Comments on 'Instruments to assess appropriateness of hip and knee arthroplasty: a systematic review'.

Osteoarthritis and cartilage·2023
Same author

Commentary on finding meaning in patient-reported outcome change scores: a seemingly unquenchable thirst for understanding.

Osteoarthritis and cartilage·2022
Same author

Cross-validation of good versus poor self-reported outcome trajectory types following knee arthroplasty.

Osteoarthritis and cartilage·2021
Same author

Examining Timeliness of Total Knee Replacement Among Patients with Knee Osteoarthritis in the U.S.: Results from the OAI and MOST Longitudinal Cohorts.

The Journal of bone and joint surgery. American volume·2020
Same author

Ninety-day and one-year healthcare utilization and costs after knee arthroplasty.

Osteoarthritis and cartilage·2019

Area of Science:

  • Genetics
  • Developmental Biology
  • Aging Research

Background:

  • The nematode Caenorhabditis elegans enters a dauer larva stage under stress conditions like overcrowding and starvation.
  • Understanding the genetic regulation of dauer formation and its relation to lifespan is crucial for aging research.

Purpose of the Study:

  • To genetically analyze mutations affecting dauer larva formation (daf mutations) in C. elegans.
  • To elucidate the genetic pathways governing dauer development and adult lifespan.
  • To identify genetic interactions that synergistically extend lifespan.

Main Methods:

  • Genetic analysis of Caenorhabditis elegans mutants.
  • Characterization of dauer formation and adult lifespan phenotypes.
  • Construction and analysis of double and triple mutant strains.

Related Experiment Videos

Main Results:

  • Mutations in daf-2 and daf-23 genes double adult lifespan, while mutations in daf-1, daf-4, daf-7, and daf-8 do not affect lifespan.
  • daf-16 mutations completely suppress the increased lifespan, and daf-18 partially suppresses it.
  • Specific combinations of daf-2 and daf-12 mutations nearly quadruple lifespan, representing a significant extension in a metazoan.

Conclusions:

  • A genetic pathway for dauer development and adult lifespan determination in C. elegans has been updated.
  • daf-2 and daf-23 play critical roles in lifespan regulation, modulated by daf-16 and daf-18.
  • Synergistic interactions between daf-2 and daf-12 offer a powerful model for studying extreme lifespan extension.