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Isolation and characterization of a new restriction endonuclease, Sru30DI, from Selenomonas ruminantium
P Pristas1, I Vanat, P Javorský
1Institute of Animal Physiology, Slovak Academy of Sciences, Kosice.
Gene
|May 26, 1995
Abstract:
The restriction endonuclease (ENase) Sru30DI, an isoschizomer of StuI, which recognizes the sequence 5'-AGG/CCT-3', was purified from a natural isolate of Selenomonas ruminatinum. The ENase was isolated from cell extracts using single-step purification by phosphocellulose column chromatography. Activity of Sru30DI is inhibited by overlapping Dcm methylation. The ENase is extremely stable at 37 degrees C and is active over a wide range of pH, temperature and salt concentrations.