Related Experiment Videos
Detection of Borna disease virus RNA in formalin-fixed, paraffin-embedded brain tissues by nested PCR
1Institute of Parasitology, Veterinary Medical Faculty, University of Zürich, Switzerland.
Journal of Clinical Microbiology
|April 1, 1995
Abstract:
A method for detecting Borna disease virus (BDV) RNA in formalin-fixed, paraffin-embedded brain tissue sections was established. By digestion with proteinase K and subsequent extraction with guanidinium thiocyanate, phenol, and chloroform, we were able to efficiently release RNA from the fixed tissues. By reverse transcription of the RNA and nested PCR a 212-bp product was generated, as expected.