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Recombination between genetically modified and unmodified Autographa californica nuclear polyhedrosis virus in
A T Merryweather-Clarke1, M Hirst, R D Possee
1NERC Institute of Virology and Environmental Microbiology, Oxford, UK.
Acta Virologica
|December 1, 1995
Abstract:
Trichoplusia ni larvae have been injected with a mixture of wild-type Autographa californica multiple nuclear polyhedrosis virus (AcMNPV) and a mutant derivative, AcRP8.UW1.lacZ, which lacks the polyhedrin gene, and has the p10 gene replaced by the Escherichia coli beta-galactosidase gene. Following plaque assay of the haemolymph and subsequent staining for beta-galactosidase activity and scoring for polyhedra, recombinant plaques were identified and the recombination frequency estimated as 6.6%.