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Characterization of cyclic AMP phosphodiesterase isozymes in rat parotid gland
A Imai1, T Nashida, H Shimomura
1Department of Oral Biochemistry, Nippon Dental University, School of Dentistry at Niigata, Japan.
Archives of Oral Biology
|February 1, 1995
Abstract:
Cyclic AMP phosphodiesterase (PDE) isozymes were isolated and characterized from the soluble fraction of rat parotid gland. Four main peaks containing PDE activity were obtained by Q-Sepharose Fast Flow column chromatography. The four peaks were identified as PDEs I-IV by kinetic properties, molecular-weight analysis and their responses to effectors and inhibitors.