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Persistence of multilineage host haemopoiesis following allogeneic bone marrow transplantation
C M Price1, S M Colman, E J Kanfer
1Leukaemia Research Fund Centre, Institute of Cancer Research, London.
The survival of host cells following high-dose cytotoxic therapy and allogeneic marrow transplantation has been established previously, but the identity of these cells has not been elucidated in detail. Four patients who received sex-mismatched marrow have been studied for up to 12 months post-transplant using a simultaneous immunophenotyping/fluorescence in situ hybridization technique. The results demonstrate residual host T cells (CD3+), B cells (CD22+) and myeloid cells (CD11c+ and CD13+), and additionally cells of progenitor cell phenotype (CD34+). The long-term persistence of host haemopoiesis may have major relevance to the post-transplant complications of marrow rejection, graft-versus-host disease, and malignant relapse.
The survival of host cells following high-dose cytotoxic therapy and allogeneic marrow transplantation has been established previously, but the identity of these cells has not been elucidated in detail. Four patients who received sex-mismatched marrow have been studied for up to 12 months post-transplant using a simultaneous immunophenotyping/fluorescence in situ hybridization technique. The results demonstrate residual host T cells (CD3+), B cells (CD22+) and myeloid cells (CD11c+ and CD13+), and additionally cells of progenitor cell phenotype (CD34+). The long-term persistence of host haemopoiesis may have major relevance to the post-transplant complications of marrow rejection, graft-versus-host disease, and malignant relapse.