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Direct evidence of a heterotrimeric complex of human interleukin-4 with its receptors
R C Hoffman1, B J Castner, M Gerhart
1Immunex Corporation, Seattle, Washington 98101, USA.
Protein Science : a Publication of the Protein Society
|March 1, 1995
Abstract:
The mode of binding of interleukin-4 (IL-4) to its two known receptors, specific receptor IL-4R and a shared receptor gamma c, was investigated using gel filtration and gel electrophoresis. A ternary complex between IL-4 and the soluble domains of the two receptors was shown to exist in solution. The association constant between gamma c and the stable complex of IL-4/sIL-4R is in the millimolar range, making the ternary complex a feasible target for crystallization studies.