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Measuring Granulocyte and Monocyte Phagocytosis and Oxidative Burst Activity in Human Blood
Published on: September 12, 2016
Ginseng-enhanced oxidative and phagocytic activities of polymorphonuclear leucocytes from bovine peripheral blood and
1Department of Veterinary Medicine, College of Animal Sciences, Zhejiang Agricultural University, People's Republic of China.
Abstract:
This study investigated the effect of a dried ginseng extract on polymorphonuclear leucocytes (PMNL) in bovine blood and milk. In a test for chemiluminescence (CL), PMNL were pre-incubated in ginseng solution at 37 degrees C in 5% CO2 for 60 min, and then stimulated with bovine serum opsonized zymosan. The CL was about 30% higher for the cells pre-treated with ginseng solutions 100-1,000 micrograms/ml as compared with the non-ginseng-treated cells. In a test for phagocytosis, PMNL and fluorescent microspheres were incubated with ginseng in RPMI-1640 supplemented with 10% bovine serum at 37 degrees C for 60 min. The proportion of actively phagocytic cells in the ginseng-treated group was greater than that in the non-ginseng treated group.
