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Related Experiment Videos

Taste bud cell generation in the perihatching chick

D Ganchrow1, J R Ganchrow, R Gross-Isseroff

  • 1Department of Anatomy and Anthropology, Sackler Faculty of Medicine, Tel Aviv University, Ramat Aviv, Israel.

Chemical Senses
|February 1, 1995
PubMed
Summary

Chick taste bud development involves cell proliferation after embryonic day 19 (E19). Early taste bud primordia (E17-19) form from quiescent cells, with significant DNA synthesis occurring post-hatching (H2).

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Area of Science:

  • Developmental biology
  • Histology

Background:

  • Chick taste bud primordia emerge late in gestation (embryonic day 17).
  • Understanding cell proliferation dynamics is crucial for taste bud development.

Purpose of the Study:

  • To investigate DNA synthesis and cell proliferation in chick taste buds from embryonic day 17 (E17) to the second day post-hatching (H2).
  • To determine the timing of cell proliferation relative to taste bud primordium formation.

Main Methods:

  • In ovo injection of tritiated thymidine during embryonic development (E15-E18).
  • Light microscopic autoradiography of anterior mandibular oral epithelium.
  • Analysis of labeled cells (DNA synthesis) and taste bud diameter.

Main Results:

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  • Significant DNA synthesis in taste bud cells primarily occurs after embryonic day 19 (E19), regardless of thymidine injection timing.
  • Early taste bud primordia (E17-19) appear to assemble from quiescent or postmitotic cells.
  • Cell proliferation is evident in all examined taste buds from E20 to H2.
  • Injection timing significantly impacts the number of labeled cells, with later injections (E17-18) yielding more labeled cells per bud.

Conclusions:

  • Chick taste bud formation involves an initial assembly phase of quiescent cells followed by a proliferative phase.
  • The timing of cell proliferation is critical, with significant activity occurring post-E19.
  • Further research is needed on the sources of cells contributing to taste bud growth.