Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Mucin coding sequences are remarkably conserved

M Verma1, M Madhu, C Marrota

  • 1Department of Biochemistry and Molecular Biology, Georgetown University School of Medicine, Washington, D.C. 20007, USA.

Cancer Biochemistry Biophysics
|April 1, 1994
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Incidence of nosocomial pneumonia and clinical outcomes of patients requiring non-invasive ventilation: A systematic review and meta-analysis.

The Southern African journal of critical care : the official journal of the Critical Care Society·2026
Same author

Conformational landscapes and binding free energies of multitarget phytochemicals reveal molecular recognition mechanisms in colorectal cancer-associated proteins.

SAR and QSAR in environmental research·2026
Same author

Soil moisture estimation using Sentinel-1 SAR and field observation in the Eastern Ghats region of India.

Environmental monitoring and assessment·2026
Same author

Development of erosion hazard index-based land suitability criteria for sustainable agriculture in the Nilgiris hills of India.

Scientific reports·2026
Same author

Glutathione-deficiency promotes basal hyperinsulinemia in the insulin secreting cell line INS-1 (832/13).

Chemico-biological interactions·2026
Same author

Genetic Insight into Gorlin-Goltz Syndrome.

Indian journal of dermatology·2026

Mucin coding sequences are conserved across many species, particularly mammals, indicating functional significance. Non-repeat regions show higher conservation, suggesting important roles beyond structural repeats in mucin evolution.

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Evolutionary Biology

Background:

  • Mucins are complex glycoproteins from glandular epithelia.
  • Aberrant glycosylation of mucins occurs in cancers.
  • Cancer-associated mucins have T-cell epitopes relevant to pancreatic and breast cancer.

Purpose of the Study:

  • To investigate the evolutionary conservation of mucin coding sequences across species.
  • To identify functionally significant regions within mucin structures.

Main Methods:

  • Polymerase Chain Reaction (PCR) amplification of mucin coding sequences.
  • Hybridization analysis and determination of sequence divergence.
  • Examination of analogous sequences in various organisms (mammals, chicken, yeast).

Related Experiment Videos

Main Results:

  • Mucin-type sequences are conserved in mammals but less so in chicken and yeast.
  • Sequence divergence increases in the order: human, monkey, rabbit/rat/cow, mouse.
  • Non-tandem repeat nucleotide sequences are more conserved than flanking sequences, suggesting functional importance.

Conclusions:

  • Mucin core protein features are broadly conserved in mammals.
  • Conserved non-repeat sequences may hold functional significance.
  • The study discusses the evolutionary implications of mucin sequence conservation.