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Related Experiment Videos

Echinococcus multilocularis protoscoleces and hepatic cell activity in vitro

C Gabrion1, S Walbaum, S al Nahhas

  • 1Laboratoire de Parasitologie comparée, URA 698 CNRS, Université Montpellier II, France.

International Journal for Parasitology
|January 1, 1995
PubMed
Summary

Co-culturing liver cells with Echinococcus multilocularis parasites and mouse fibroblasts revealed that albumin production is stimulated. This suggests a complex cellular interaction involving liver cells and activated Kupffer cells.

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Area of Science:

  • Hepatology
  • Parasitology
  • Cell Biology

Background:

  • Echinococcus multilocularis is a parasitic tapeworm that causes cystic echinococcosis.
  • Hepatocytes are the primary functional cells of the liver.
  • Kupffer cells are resident macrophages in the liver, playing a role in immune responses.

Purpose of the Study:

  • To investigate the interaction between E. multilocularis protoscoleces and hepatocytes.
  • To assess the impact of parasite co-culture on hepatocyte function, specifically albumin and transferrin secretion.
  • To explore the role of co-cultured cell lines (IAR 20 or BALB/c 3T3) in modulating hepatocyte activity.

Main Methods:

  • Co-culturing E. multilocularis protoscoleces with primary hepatocytes.
  • Utilizing IAR 20 (a rat hepatocyte cell line) or BALB/c 3T3 (a mouse fibroblast cell line) as co-culture components.

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  • Measuring the secretion levels of transferrin and albumin in the culture media as indicators of hepatocyte activity.

    • Main Results:

    • Co-culturing hepatocytes with BALB/c 3T3 cells resulted in higher levels of transferrin and albumin secretion compared to co-culture with IAR 20 cells.
    • In the presence of E. multilocularis, transferrin levels remained unchanged.
    • Albumin production was significantly stimulated within the first 48 hours of co-culture with the parasite.

    Conclusions:

    • The study suggests that E. multilocularis protoscoleces can modulate hepatocyte function.
    • The observed stimulation of albumin production may involve a cooperative mechanism between hepatocytes and activated Kupffer cells.
    • This cellular cooperation mirrors responses seen in acute inflammatory reactions, highlighting a potential host-parasite interaction pathway.