Related Experiment Videos
Vibrio cholerae infection and immunity in mice
The Australian Journal of Experimental Biology and Medical Science
|October 1, 1976
Summary
A highly virulent Vibrio cholerae (V. cholerae) strain, 569B/MP, was developed for cholera research. Storing this strain at -60°C maintains its virulence for at least 9 months, aiding vaccine efficacy studies.
Area of Science:
- Microbiology
- Infectious Diseases
- Immunology
Background:
- Lyophilized Vibrio cholerae (V. cholerae) cultures lose virulence during storage.
- Maintaining V. cholerae virulence is crucial for accurate challenge models in cholera research.
Purpose of the Study:
- To isolate and characterize a highly virulent V. cholerae strain for use in challenge studies.
- To establish a reliable method for preserving V. cholerae virulence for extended periods.
Main Methods:
- Isolation of a hypervirulent V. cholerae strain (569B/MP) through passage in mice.
- Storage of the virulent strain at -60°C to assess long-term viability and virulence.
- Evaluation of the strain's pathogenicity in neonatal and adult mice.
Main Results:
- The 569B/MP strain caused rapidly fatal intestinal infections in 6-day-old mice.
- Storage at -60°C maintained the strain's high virulence for at least 9 months.
- The strain induced choleraic symptoms and mortality in 50% of adult mice upon oral challenge.
Conclusions:
- The 569B/MP strain is a valuable tool for cholera research, offering consistent virulence.
- Cryopreservation at -60°C effectively preserves V. cholerae virulence for long-term use.
- This model facilitates the assessment of cholera vaccine efficacy.